It is the first time that the Wolves’ men’s first team are playing a competitive fixture at the AOK Stadion.
Instead of their new kit, VfL will be wearing a specially made anniversary shirt – as they will in their opening league game against Bayer Leverkusen.
The Green-and-Whites have never previously faced Union Fürstenwalde, who finished fourth in the Regionalliga Nordost last season.
It is also the very first time that Union Fürstenwalde are participating in the first round of the DFB Cup.
VfL are set to play their 110th match in this competition.
The last time that the Green-and-Whites played at home in the first round of the cup was in 1990, when they were an amateur side in the third tier. They lost 6-1 at the Stadion am Elsterweg to 1. FC Köln, who had finished runners-up in the Bundesliga the previous season.